When both Sp6a and Sp6b have Sp0- replaced with Sp0+ during phase one of the Sp0 pathway, this can result in displacement of the Sp6ab structure. Sp0+ has opposite outside data to Sp0-. SD1 acceptance for SpO- encourages closer collaboration with SpO+ and both Sp6a and Sp6b before they follow their own pathway. Sp0+ involvement with both Sp6a and Sp6b during phase two of the Sp0 Pathway will continue to decrease over time.